WHAT ARE STEMCELLS? What are stem cells? We have it in our bodies、Like skin and blood、Shorter lifespan of each cell、Keeping an organization in constant rotation、It has cells that have the ability to recreate and replenish lost cells。Stem cells are cells that have this ability。To be called stem cells、The following two competencies are indispensable。One is、skin、red blood cell、platelets, etc.、The ability to produce the various cells that make up our bodies(Ability to differentiate)、The other is the ability to divide into cells that have exactly the same ability as you(Self-replicating ability)It is。 Stem cells can be broadly divided into two types。One is、Like skin and blood、In tight tissues and organs、It is a stem cell that continues to replace the disappeared cells。This type of stem cell is called tissue stem cell。Tissue stem cells can't be anything.、If it is a hematopoietic stem cell that produces blood, it is a cell of the blood system、If it is a neural stem cell that makes the nervous system, it is only a cell of the nervous system、As stated、The role is determined。Another thing is、ES Cells(Embryonic stem cells)Of so、If it is a cell of our body、It is a pluripotent stem cell that can produce any cell。 What you mean、Pluripotent stem cells、It is also possible to produce various tissue stem cells in our bodies。What are induced Pluripotent Stem Cells (iPS cells)?、It is a pluripotent stem cell artificially created from ordinary cells。Taking advantage of these properties of stem cells,、Research on a new treatment method called regenerative medicine, which uses cells themselves as medicines to cure injuries and diseases,、Research is underway to reproduce the state of cells in the body outside the body to investigate the mechanism of disease。 Aging care with cytokines in stem cell culture medium Stem cells produce proteins that promote cell proliferation and differentiation according to the environment。These proteins are called cytokines。Cytokines act as signaling substances between cells.、It plays an important role in the functional restoration of damaged tissues and cells in the body。Communicate between cells、Cells that receive cytokines begin to work。 The aging phenomenon is、The tissues and cells of the body are damaged by various causes.、It is caused by the accumulation of such a situation that it is not possible to fully recover。Regenerate damaged body tissues and cells、Restoring lost functionality is、It can be said that it is a very effective aging care。By awakening dormant cells by cytokines、It is expected to exert a powerful anti-aging effect。 What is a stem cell culturist who is attracting attention Stem cell culture is a technology that is attracting great promise in various fields such as drug development and regenerative medicine。There are a wide range of sites that require cell culture technology in this field, which has a lot of potential in the future.、Culturists who specialize in these are currently attracting attention。What is a culturist?、In the medical and research fields、He is an expert in culturing stem cells and using them for various experiments and treatments。Stem cells、High cell differentiation capacity、Because it can differentiate into various types of cells、There are high expectations for regenerative medicine and treatment development。As a result、The technology for culturing stem cells is、It is one of the most important technologies in the medical and life sciences fields。Specifically、Stem Cell Culturists、Creation of media for culturing stem cells、Optimization of incubation conditions、Regulation of cell differentiation and proliferation、Cell testing and analysis、Management of cultured cells, etc.、Perform a series of tasks required for stem cell culture。Also、Stem cells、Because it has different properties from normal cells、Removal of abnormal cells that occur during culture and、Prevention of infectious diseases, etc.、It is also necessary to have the knowledge and skills to deal with the technical difficulties of cell culture。Stem cell culturists include:、A high level of expertise and skills in stem cell culture technology is required。As a result、Learn the basics of stem cell handling techniques and cell culture at universities and vocational schools.、Laboratories and pharmaceutical companies、Human resources with practical experience in hospitals and other institutions are active as stem cell culturists。Let's ask Mr./Ms. Yamaguchi, the chief culturist of Biostyle Clinic, about the culturists who attract attention in this way。 Biostyle Clinic Chief Culturist Hitoshi Yamaguchi / Yoshichika Yamaguchi Education：Graduated from the Department of Biotechnology, Faculty of Engineering, Kansai University (Laboratory of Cellular Engineering)：Ibaraki City, Osaka Prefecture Career：Kishimoto Institute of Medical Science, Heavenly Therapy Tokiwakai Tokiwa Hospital (Immunology Division, Detailed Culture Room)、After working at the Japan Culture Research Institute, he is currently concurrently serving as a SCCL (Head of Culture Technology at SCC Lab) and Chief Culturist at BIOSTYLE CLINIC Hobbies：Pets (all animals and plants)、Motorcycle (blank 10 years) Why did you decide to become a culturist Why did you choose the profession of culturist? I have loved living things since I was a child, and I wanted to learn about biotechnology related to them.、At university, I went on to the Department of Bioengineering and studied plant cells to develop a propagation method for red beans。After graduating, I got a job at a major medical company and was engaged in clinical laboratory work.、When there was a recruitment for a culturist at the hospital after changing jobs,、I decided to pursue a career as a culturist, which I had been interested in for a long time。At that hospital, we mainly cultured self-activating lymphocytes and autologous fibroblast cultures.、Since we did not have special equipment, we had to do research by hand from tissue collection to culture, which was very difficult。After that、Another laboratory offered me to make a stem cell culture supernatant.、Utilizing the cell culture know-how cultivated at the hospital, we have refined the technology through trial and error of stem cell culture, and have conducted research for various treatments。My career as a culturist is about 13 years。In this industry, treatment using cultured solutions derived from fat and dental pulp stem cells has become the mainstream.、Current、Umbilical cord blood、amnion、placenta、By utilizing cells such as bone marrow、We are researching new stem cell culture supernatants that can achieve higher performance。 The difficulty of culturing stem cells and the commitment of professionals It is said that stem cell culture is very difficult.、What are the difficulties? The biggest difficulty in culturing stem cells is:、It is about balancing cell proliferation and differentiation。Basically, we make a time schedule and cultivate it.、Because cells are not always in the same state、It is unlikely that things will go as planned。For example, in the morning and in the evening, the state of the cells is completely different。Therefore, while constantly observing the shape of the cells through a microscope, we fine-tune the amount and timing of the addition of the medium。If it grows too quickly, it will cause a lack of nutrients and growth space in the medium.、Cell proliferation stops and cell deterioration begins。On the other hand, if the proliferation is slow, the number of cells will not increase sufficiently.、Unable to obtain sufficient supernatant。Also、Culturing stem cells、It is not enough to have the knowledge and skills necessary for cell culture.、Always pay attention to new research and technology trends.、We believe that it is necessary to continuously learn the latest information and methods。 Please tell us about your professional commitment because the cells we work with are ultimately used by end users in medical settings.、Very high quality is required。As a result、Cultivation is carried out under a strict quality control system.、It is required to provide a supernatant that is safe and effective。Also、Accurately grasp the state of cells in culture,、It is also important to provide an optimal environment。For that、The amount of protective agent used to freeze and thaw cells and the time of use、Periodic cytological examinations and measurement of cell activity、Thorough management of media and culture equipment, etc.、It is required to provide a supernatant of stable quality。In addition、While we are cells alive、I think it's important to treat them politely。Carefully cultured by hand、Constantly check the condition and growth of cells,、By providing optimal conditions tailored to individual cells、We believe that we can produce high-quality supernatants。 What about the attention-grabbing three-dimensional culture? In general, cell culture is done in two dimensions with flasks.、Three-dimensional culture is one of the technologies that has recently attracted attention in the field of stem cell research。The benefits of three-dimensional culture are wide-ranging.、The biggest benefit is、It is the ability of cells to grow in a natural way。In three-dimensional culture、Cells are different from conventional two-dimensional cultures.、It grows in clusters in spherical clusters or sheets。By growing cells in three-dimensional space、It can mimic an environment that more closely resembles a real organization。Stem cells cultured in such an environment、More likely to produce more accurate experimental results、Analyze cell functions and behavior more accurately。Also、Three-dimensional culture、Cell-cell interactions、Cell-substrate interactions、Oxygen supply、Metabolite excretion、The process of cell growth and differentiation, etc.、It considers the complex interactions between cells and their surroundings。For this reason、Three-dimensional culture、It is the basis of a tissue engineering approach to modulating stem cell differentiation。 However,、There are also some disadvantages of three-dimensional culture。The biggest disadvantage is、It is difficult to accurately position cells in three-dimensional space。Also、In three-dimensional culture, cell agglomeration can be formed.、The diffusion of nutrients and oxygen is not uniform on the surface and inside of the agglomerate。By this、Cells are overstressed,、Can cause cell death and abnormal growth。As a result、As a culturist、Cell type and media、Support materials, etc.、It is necessary to adjust various conditions appropriately, and very advanced technology is required。 培養士という仕事において最もやりがいを感じる瞬間は何でしょうか？ やりがいを感じる瞬間は、It's time to work on new research that could lead to stem cell-based treatments。Stem cells、It has the ability to self-replicate and differentiate.、It is expected in various fields such as regenerative medicine and drug discovery research。Our research、The moment when you realize that it has the potential to contribute to society in the future、It's very rewarding。Also、Great care in working with stem cells、It is necessary to proceed with the experiment carefully。As a result、By proceeding with the experiment with the utmost care,、There is also a sense of accomplishment that we can proceed with safe research。The most rewarding moment is still、That's when I heard the voice of an end user who used the stem cell culture solution produced from my cultured cells that he was cured。These experiences are a great encouragement for me to work on my work。 What are your views on cosmetics containing stem cell culture supernatant, which has been attracting attention in recent years? In the field of cosmetics, stem cell culture supernatant is receiving the most attention。This liquid is、Cell growth factors and cytokines、Enzyme、protein、and micronutrients, etc.、It is said to be effective for beautiful skin and anti-aging。For this reason, many cosmetics containing stem cell culture supernatant are developed by various manufacturers。There are many types of cosmetics that contain stem cell culture supernatant。For example、lotion、Serum、Cream、puck、There are masks and so on。These products are、We respond to various skin problems.、Especially the elasticity and firmness of the skin、And it is said to be effective in transparency。 However, most of the cosmetics currently on the market containing stem cell culture supernatant、The concentration and type of stem cell culture supernatant are not clearly stated.、Because it is likely to be in a poorly preserved environment、To be honest, I'm skeptical when it comes to effects。Therefore, we believe that it is important to select cosmetics that have introduced technologies that avoid direct sunlight and high temperatures and humidity, which are natural enemies of stem cell culture supernatant。 The development of cosmetics containing stem cell culture supernatant by cosmetics manufacturers、It is expected that it will continue to advance in the future。However,、It is necessary to provide accurate information to consumers.、How to use it according to the individual's skin type and how to store it correctly are required.、It can be said that it is necessary to be constantly aware of it。 Please tell me the precautions for storing stem cell culture supernatant Stem cell culture supernatant、It is stored in two forms: liquid and freeze-dried。Since each is handled differently, it is necessary to store it appropriately according to the form。 In the case of liquids、Must be kept refrigerated。Short shelf life、Temperature changes and vibrations、Due to the susceptibility to light and water、When moving liquids, it is necessary to handle them gently。In general, cosmetics containing stem cell culture supernatant are liquid.、The quality will change depending on the state of preservation.、First of all, purchase from a reliable dealer、And after purchasing, store it in a place away from direct sunlight, high temperatures and humidity, and use it up immediately after opening。 For freeze-drying、Due to the stability of the protein、Can be stored at room temperature。Due to the absence of preservatives and stabilizers、Add water just before use to dissolve。However、Even in freeze-dried conditions、Because it may deteriorate depending on the storage environment、It is important to maintain the right temperature and humidity。In the case of freeze-drying、Due to the possibility of long-term storage、It plays an important role in research and product development。 What are your thoughts on the future of stem cell culture supernatant? Stem cell culture supernatant、It is expected to be applied in various medical fields。For example、In the field of aesthetic medicine、Cosmetics and medical supplies containing stem cell culture supernatant、Skin and hair regeneration、Rejuvenation、It is used for wound treatment。Also、Research is also underway on treatments using stem cell culture supernatant.、It is expected to be applied in the field of regenerative medicine。 Specifically, in what medical fields are it expected to be applied? Stem cell culture supernatant、It is expected to be applied in a wide range of medical fields。For example、It is expected to be used in regenerative medicine for diseases such as myocardial infarction and stroke。Also、It is also expected to be applied in the treatment of arthritis and liver disease。In addition、Stem cells may also be used for cancer treatment and immunotherapy.、It is expected that stem cell culture supernatant will be used in this process as well。 How are the treatments performed in medical facilities? As a method of treating stem cell culture medium、Although there are intravenous injections and subcutaneous injections,、I recommend subcutaneous injections。Intravenous injections intensify metabolism、Because it is easy to cause fever、This is because subcutaneous injections are more effective。Subcutaneous injections、Dissolve in saline、2Inject an appropriate amount from ~5cc。Subcutaneous injections、By the method of giving injections into the subcutaneous tissue located under the skin、Less painful than intramuscular injections、The absorption rate is also slow。However,、For the preparation to be stationed in place、Symptoms of temporary swelling may occur。 Can you tell us more about its application in the beauty field? Stem cell culture supernatant、Due to the presence of unique ingredients、It can be expected to have a high beauty effect that is different from conventional cosmetics。Promotes skin turnover、Improves blemishes and wrinkles、It is also effective in treating wounds.、Calms inflammation、By promoting regeneration、Due to its healing effect、Cosmetics containing stem cell culture supernatant have been attracting attention in recent years。However、For the development of cosmetics containing stem cell culture supernatant、It is also true that a high level of technical skill is required。How to cultivate stem cells and how to produce culture supernatant、It's very complicated.、It also requires strict quality control。In addition、Cosmetics containing stem cell culture supernatant、Effects and side effects on the human body also need to be fully studied。What you mean、Stem cell culture supernatant、Also in the medical field、Also in the field of beauty、It can be said that it is an ingredient with great potential。However、For its development and use,、It requires high technical skills and sufficient research.、Efforts to ensure safety and effectiveness are essential。Attention will continue to be paid to the progress of stem cell research.、Always from an ethical point of view.、It will be required to be used safely and effectively。 Stem cell culture supernatant、It is an innovative beauty ingredient developed by the advancement of stem cell research.、It is expected to attract more and more attention in the future。However,、We must not forget that there are many researchers and technologists behind it。What they strive for every day、It is connected to our beauty and health。 Cell Processing Culture Room Stem Cell Culture Supernatant Biostyle Clinic Ginza (formerly Ai Medical Clinic) ACCESS :7-13-12 Ginza, Chuo-ku, Tokyo Success Ginza 7 Building 11th Floor TEL:0120-910-808 https://biostyle.clinic/ Biostyle Clinic Kobe Sannomiya (formerly Ai Medical Clinic Kobe) ACCESS :2-1-4 Nunobiki-cho, Chuo-ku, Kobe-shi TAKAI BLD 3rd floor TEL:0120-930-039 https://biostylekobe.clinic/ ...
